Coding
INPNC-EcAK

Part:BBa_K3332007

Designed by: Xiaoyu Wang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 20:05, 17 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


INPNC-GOX

We anchor GOX protein onto membranes through INPNC to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
    Illegal NgoMIV site found at 1488
    Illegal AgeI site found at 1558
    Illegal AgeI site found at 1635
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None