Part:BBa_K3504012

MiRNA FF4

Part Description

MiRNA FF4 is a micro RNA that has the ability to repress transcription. MiRNA FF4 is one of microRNAs that were constructed according to the pPRIME approach with stem-loops of functional sequence cloned into miR-30 backbone and fused into 3’-UTR of CMV-driven neomycin resistance gene. Their names are identical to their parent siRNAmolecules, i.e., FF3, FF4, FF5 and FF6

Usage

Genetically encoded miRNA sensors (which sense a solitary miRNA binding) and cell classifiers (which sense different miRNA inputs at the same time) can give data about infection state, activate reactions in cells explicitly communicating either a healthy or diseased miRNA profile. Through analyzing and studying multiple synthetic miRNAs and testing them, we found that miRNA FF4 shows high potency in translational silencing and significant cross-talk in the tumor micro-environment, accordingly we modified our OFF-switch to contain miRNA FF4 target site in our circuit in the 5’ UTR OFF position. Once miRNA FF4 binds to its target site on the mRNA in the 5’ UTR OFF position, this triggers a series of post-transciption modifications that regulate the gene expression and transcription of the used SFV replicon.

Improvements

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]