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Part:BBa_K3505006:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-11)
Revision as of 16:24, 11 October 2020 by Registry (Talk | contribs)

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Tyr1-AIDAc Tyrosinase fused to membrane protein AIDAc. GB compatible B3-B5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1439
    Illegal EcoRI site found at 2135
    Illegal XbaI site found at 1043
    Illegal PstI site found at 1487
    Illegal PstI site found at 2073
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1439
    Illegal EcoRI site found at 2135
    Illegal PstI site found at 1487
    Illegal PstI site found at 2073
    Illegal NotI site found at 1211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1439
    Illegal EcoRI site found at 2135
    Illegal BglII site found at 471
    Illegal BamHI site found at 454
    Illegal BamHI site found at 2337
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1439
    Illegal EcoRI site found at 2135
    Illegal XbaI site found at 1043
    Illegal PstI site found at 1487
    Illegal PstI site found at 2073
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1439
    Illegal EcoRI site found at 2135
    Illegal XbaI site found at 1043
    Illegal PstI site found at 1487
    Illegal PstI site found at 2073
    Illegal AgeI site found at 533
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GB asteria


Source

Tyr1 from Rhizobium etli in the supplementary of [3] AIDA from E.coli in the supplementary of [2]

References