Coding

Part:BBa_K3504007

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-09-19)
Revision as of 18:44, 9 October 2020 by Ahmed Wael (Talk | contribs) (→‎Improvements)


Gly-Ala repeats

Part Description

Glycine-Alanine repeat (GAr) sequence of the Epstein-Barr virus-encoded EBNA-1 prevents presentation of antigenic peptides to major histocompatibility complex class I molecules. This has been attributed to its capacity to suppress mRNA translation in cis.

Usage

Characterization

Improvements

This part was improved by changes in its sequence that allowed it to further protect the circuit.

Figure 1. Optimized Gly-Ala repeats.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 262
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 442
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 571
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None