Regulatory

Part:BBa_K3487007

Designed by: Jianhua Zhou   Group: iGEM20_SZPT-CHINA   (2020-10-09)
Revision as of 16:24, 9 October 2020 by Registry (Talk | contribs)

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Lysine riboswitch

Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256


[edit]
Categories
//rna/riboswitch
Parameters
None