Coding

Part:BBa_K3621002:Design

Designed by: Sangita Vasikaran   Group: iGEM20_MIT   (2020-10-04)
Revision as of 14:02, 4 October 2020 by Registry (Talk | contribs)

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Cas6-> cleaves cut site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NotI site found at 71
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 490
    Illegal PstI site found at 514
    Illegal NgoMIV site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ERN crosstalk


Source

Sequence

References