Coding

Part:BBa_K3349001:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 14:57, 23 September 2020 by Kristiturton (Talk | contribs) (→‎References)


Pectin Lyase PelB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 261
    Illegal BamHI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus based on previous pectin lyase studies [1]


Source

The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.

References

1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from Bacillus haldurans M29.