Composite
Part:BBa_K2916041:Design
Designed by: Konstantinos Ragios Group: iGEM19_EPFL (2019-09-26)
Revision as of 03:00, 22 October 2019 by Konstantinos (Talk | contribs)
Expression of RF2 in E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1245
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 681
Illegal BglII site found at 1138 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein was expressed in pET15b in BL21(DE3) E.coli strain.
Source
Sebastian Maerkl Lab at EPFL, Switzerland.
http://www.addgene.org/124130/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568
Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.