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Part:BBa_K3093012:Design

Designed by: Liu Xu   Group: iGEM19_ECUST_China   (2019-10-16)
Revision as of 02:47, 22 October 2019 by Still Water (Talk | contribs) (→‎References)

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HlyA-tag (OmpT-cleavable linker)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

F-primer: ggtagcgcacgacgagcaggcggtggcggttctttagcctatggaagtcaggg R-primer :cgcctgctcgtcgtgcgctaccgccaccgccagcaccggtggagtgacgac


Source

References

[1]Kramer RA, Zandwijken D, Egmond MR, Dekker N. In vitro folding, purification and characterization of Escherichia coli outer membrane protease ompT. Eur J Biochem. 2000 Feb;267(3):885-93. PubMed PMID: 10651827.