Composite

Part:BBa_K098988:Design

Designed by: Harvard iGEM 08   Group: iGEM08_Harvard   (2008-10-26)
Revision as of 20:46, 26 October 2008 by Registry (Talk | contribs)

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temperature sensitive cI inducible system with GFP reporter and high promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1600


Design Notes

This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site.

The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]


Source

PGW7 plasmid and Biobricks parts.

References