Part:BBa_K1692032
amilCP blue chromoprotein with RBS and promoter
This plasmid contains the amilCP blue chromoprotein gene found in part BBa_K592009 and the RBS and promoter from part BBa_K608002. For more information about this gene, please refer to the BBa_K592009 part page.
Usage and Biology
The chromoprotein amilCP is part of a family of GFP-like fluorescent proteins derived from reef-building corals of the class Anthozoa (Alieva et al., 2008). Similar to the GFP family of proteins, the β-barrel of amilCP encloses a chromophore (Tafoya-Ramírez et al., 2018). Along with other coral chromoproteins, amilCP is non-fluorescent and forms a tetramer, resulting in a fairly stable protein structure (Alieva et al., 2008; Tafoya-Ramírez et al., 2018). These GFP-like fluorescent proteins give corals their vivid and varied colors (Alieva et al., 2008).
Isolated from the species Acropora millepora, amilCP was first described in 2008, and is characterized by a strong color due to its very high molar extinction coefficient of 87 600 (Alieva et al., 2008). Its maximum excitation wavelength is 588nm (Alieva et al., 2008). Interestingly, amilCP’s max absorption is shifted into the red spectrum by ~10nm (592nm), hence appearing more blue than purple to the naked eye (Alieva et al., 2008). Such blue color is due to 2 mutations resulting in amino acid substitutions (Alieva et al., 2008).
References<b></p>
<p>Alieva, N. O., Konzen, K. A., Field, S. F., Meleshkevitch, E. A., Hunt, M. E., Beltran-Ramirez, V., … Matz, M. V. (2008). Diversity and evolution of coral fluorescent proteins. PLoS ONE, 3(7). https://doi.org/10.1371/journal.pone.0002680 </p>
<p>Tafoya-Ramírez, M. D., Padilla-Vaca, F., Ramírez-Saldaña, A. P., Mora-Garduño, J. D., Rangel-Serrano, Á., Vargas-Maya, N. I., … Franco, B. (2018). Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection. Molecules (Basel, Switzerland), 23(6). https://doi.org/10.3390/molecules23061328 </p>
Sequence and Features
Illegal NheI site found at 30
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