Composite

Part:BBa_K3016210:Experience

Designed by: Antti Pennanen, Antti Toivanen, Amanda Raitosalo   Group: iGEM19_Aalto-Helsinki   (2019-10-16)
Revision as of 19:04, 21 October 2019 by Toivanen (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3016210

Use by Aalto-Helsinki 2019

Aalto-Helsinki attempted to characterize this part in Vibrio natriegens by cloning and expressing it. We were unsuccessful as we found the CDS always mutated during the cloning phase. We used a vector with a low copynumber p15A ori and a tight pRhaB promoter, with which we had no problems when working with other genes (YGFP and TfoX). This implies that the gene is extremely toxic to Vibrio natriegens cells.


User Reviews

UNIQ96eb047988e7417a-partinfo-00000000-QINU UNIQ96eb047988e7417a-partinfo-00000001-QINU