Part:BBa_K3081052

Quorum Sensing CRISPRri System Enables Spatial-level Growth Control and Ultrasensitive Autoregulation of Growth

Based on the well-characterized growth control system, we constructed a quorum sensing CRISPRri system (qs-CRISPRri) to realize smart regulation of bacterial overall states. At first, we use GFP as the output to test our system.

Figure 1.The donor-receiver split quorum sensing system of CRISPRri system. Left figure is the sketch map of how donor and receiver bacteria is dropped onto the agar plate. Right figure is the real picture of agar plate under illumination of blue light. The location of the white arrow is the donor cells. Receiver cells are marked by number one to six.

A time-scale quorum sensing system can be transformed to spatial-level through a donor/receiver system (Figure 1). The donor cells, which merely express and release AHL, would activate the GFP expression of receiver cells through AHL diffusion. This is validated on the agar plate, by dropping the donor cells in the center and receiver cells around them with different distances. We found a progressive decrease in fluorescence as the receiver cells locate farther from central AHL donor.