Part:BBa_K3046001

PLEAPglaA_2

This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project ===Usage and Biology=== parts.igem.org ===Usage=== This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.

Characterization

This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger.The consensus promoter was expected to have really high expression. This version has “noise" added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.
This promoter was characterised using an mCherry test device,<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3046009" target="_blank">BBa_K3046009</a>, inserted into an AMA1-based test plasmid, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021" target="_blank_">BBa_K3046021</a>, and characterisation was done in multiple scales using a confocal microscope, a microbioreactor (BioLector, m2p-labs) for microtiter scale, in shake flasks for medium scale and in a 1 liter bioreactor for large scale.

The microtiter scale cultures were made by inoculating 10⁷ spores in 1.5 mL minimal media,

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 50
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]