Translational_Unit

Part:BBa_K2999001

Designed by: Jinshui Lin   Group: iGEM19_YAU-China   (2019-10-14)
Revision as of 14:05, 21 October 2019 by Zhangheng (Talk | contribs)


RBS+PslG

This part is mainly used for the encoding extracellular polysaccharide hydrolase PslG and the hydrolysis of such extracellular polysaccharides of Pseudomonas aeruginosa PAO1 biofilm . The first 50 bp of the sequence is its ribosome binding site. For compatibility, 254th nucleotide G was changed to A (same as leucine) and 1358th nucleotide G to A (same as leucine).

Usage and Biology

Please click:BBa_K2999009


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 356
    Illegal XhoI site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 660
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None