Composite

Part:BBa_K3206014

Designed by: Karen Fung   Group: iGEM19_Newcastle   (2019-10-16)
Revision as of 12:18, 21 October 2019 by Karen98 (Talk | contribs)


His-tagged eGFP under Bba_J23100 Promoter

The part BBa_K3206012 has been designed to be under the promoter Bba_J23100. Purified protein can then be used to formulate a standard curve of the fluorescence level of the protein concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 777
    Illegal BamHI site found at 783
    Illegal BamHI site found at 789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Newcastle 2019 Characterisation

[edit]
Categories
Parameters
None