Part:BBa_K2957043
CMV-2xGal4 (Gal4 repressible promoter)
Contents: CMV-2xGal4 (Gal4 repressible promoter)
What is it?: A Basic part; it is a promoter and allows for inducible expression of a gene. This particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)
What does it do?: This promoter is only active when induced by the syn-notch receptor and was put in our genetic circuits to show inducible production of the chemokines we were testing. This part was important to show a wave mechanic since when neutrophils expressing the syn-notch antigen would bind to the receptor on HEK cells, the promoter can activate more or less chemokine production
How to use it?: You can use this part for Type IIS Cloning. It is usually found in a plasmid with BsaI cut sites that allows for this. With other Basic parts like pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the promoter part alone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 92
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 92
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 92
- 1000COMPATIBLE WITH RFC[1000]
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