Plasmid_Backbone
pMA-BBFR

Part:BBa_K157000:Design

Designed by: Raik Gruenberg and iGEM Team Freiburg   Group: iGEM08_Freiburg   (2008-10-24)
Revision as of 10:32, 24 October 2008 by Kristian (Talk | contribs) (→‎Design Notes)

pMA-BBFR - plasmid description


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2400
    Illegal suffix found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2400
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 2406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2400
    Illegal suffix found at 11
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Plasmid containing BioBrick 3.0 (Prefix and Suffix for protein fusion)Restriction sites

Source

Plasmid designed by Raik Groenberg

References