Composite

Part:BBa_K3088001

Designed by: Syailendra Karuna Sugito, Elvan Wiyarta, Eko Ngadiono, Refael Alfa Budiman, Aditya Parawangsa   Group: iGEM19_UI_Indonesia   (2019-10-07)
Revision as of 08:52, 20 October 2019 by Enga2 (Talk | contribs) (→‎Usage and Biology)


HBEGF/ENVZ Chimeric Complete

Heparin-binding EGF-like growth factor (HB-EGF) is a unique receptor of the diphtheria toxin. EnvZ, on the other hand, is a member of two-component regulatory system that is important for bacteria osmoregulation. When diphteria toxin binds to HB-EGF, it will phosphorylate EnvZ and activates the signalling cascade pathway. Once EnvZ is phosphorylated, OmpR (a transcription factor of OmpC promoter) will also be phosphorylated. The phosphorylated OmpR will initiate OmpC transcription and express the gene following OmpC promoter. This is the complete part containing the appropriate promoter T5 lac promoter (BBa_K592008), Ribosomal Binding Site (BBa_B0034), our HBEGF/EnvZ chimeric protein (BBa_K3088001), Histidine tag (BBa_K1223006), and double terminator (BBa_B0010 and BBa_B0012)

Usage and Biology

This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.

Sequencing of this part (BBa_K3088001) combined with part (BBa_K769001) is confirmed and showed as follow:

Figure 1. This the illustration of the plasmid (pSB1C3) containing part BBa K3088001 and BBa_K769001.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None