Part:BBa_K2957022

TRE, IL-8 (CXCL-8) with NeonGreen tag (C-terminal)

Contents: TRE, IL-8 (CXCL-8), NeonGreen tag, SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for inducible IL-8 expression.

What does it do?: It's responsible for the expression of the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It has a NeonGreen tag attached.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL-8. See results below + see more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1399
  Illegal PstI site found at 850
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 850
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 624
  Illegal BamHI site found at 866
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1399
  Illegal PstI site found at 850
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1399
  Illegal PstI site found at 850
  Illegal AgeI site found at 1405
  
• 1000
  COMPATIBLE WITH RFC[1000]