Coding

Part:BBa_K2969000

Designed by: Ziyi Zhao   Group: iGEM19_UCAS-China   (2019-09-01)
Revision as of 15:23, 19 October 2019 by Aster (Talk | contribs)

TEV protease

TEV protease is a highly specific cysteine protease from Tobacco Etch Virus that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. In our project we insert the recognition sequence into the transcription factors so that the expression of protease can inhibit the action of transcription factors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 667


[edit]
Categories
Parameters
None