Part:BBa_K3182009

Parseq-α

Parseq alpha is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea do to there hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptide in different concentration. they were then incubated at 37°C with shaking every 15 minutes. the plate was incubated for 16 hours and OD⁶⁰⁰ was measured. The results from this can be seen in Figure 1.. Parseq alpha showed an inhibiting effect on the growth of E.coli. 30 ng/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.

Figure 1 Shows the antimicrobial activity of Parseq Alpha on A) E.Coli. and B) B.subtilis. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C. shaking was conducted for 1 minute every 15 minutes and the OD⁶⁰⁰ were messured every hour for 16 hours. the negative control represents the bacterial growth with no antimicrobial peptides precent. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]