Tag

Part:BBa_K3187018

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 11:33, 19 October 2019 by Lertu (Talk | contribs)


PolyG Tag (GGGG) for Sortase-mediated Ligation

Profile

Name PolyG-tag
Base pairs 12
Molecular weight 300 Da
Origin Synthetic
Properties second sequence for a Sortase A mediated reaction

Usage and Biology

The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif (BBa_K3187019) and a N-terminal poly glycine motif [1] [2] [3] . The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.

References

  1. ↑ Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering [1]
  2. ↑ Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation [2]
  3. ↑ Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering [3]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None