Coding

Part:BBa_K3195003:Design

Designed by: JingYuan Liu   Group: iGEM19_SEU-Nanjing-China   (2019-09-23)
Revision as of 07:59, 19 October 2019 by Bread (Talk | contribs) (→‎References)

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His-tagged Cathepsin B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 369
    Illegal XhoI site found at 958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because Cathespin B gene is in eukaryotes，we optimized its condons for better prokaryotic expression.Considering the following seperation and purification,we add 6X His at its tail.In addition,we cut off the signal peptide.

Source

Cathespin B gene from Branchiostoma belcheri tsingtauense

References

[1]He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B http://doi.org/10.1098/rspb.2018.0438