Part:BBa_K577881
pC+AraC+pBad+GFPc
This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a GFP gene. The whole part is flanked by the regular E, X and S, P restriction sites, so it can be approached with the usual restriction digest method. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the GFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1247
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1187
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1923
Illegal SapI site found at 961
XHD-WS-Wuhan-B 2019's Characterization
Here, we used K577881 part, an inducible araC-pBAD promoter with GFP , to investiagte effect on different concentrations of arabniose on GFP expression in this part, and to provide a more detailed data for this part. 1. Growth curve of E.coli containing part K577881 in different concentration of L-arabinose As shown in Fig.1, the growth of bacteria was not influenced at the range of 500 -10000μM of L-arabinose. When the concentration of L-arabinose reached 50000mΜ, a little inhibition was observed, indicating a little toxicity to cells at this level. Fig.1
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