Composite

Part:BBa_K3187015

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 19:13, 18 October 2019 by PeterGockel (Talk | contribs)


Constitutively expressed chromoprotein amilGFP with Translation Enhancing 5'-UTR

Improved Version of BBa_K1073024

BBa_K1073024 encodes the yellow chromoprotein amilGFP derived from the coral Acropora millepora [5] under the control of a strong constitutive promoter (BBa_J23100) in combination with a ribosomal binding site ( BBa_B0032). The improved and the original version of the part were cloned into the pSB1C3 backbone and transformed in E. coli BL21 (DE3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None