Part:BBa_K3160021

pEGFP-miR196a-148a sensor

The expression of miR-196a and miR-148a were found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a miR-148a sensor contain two complementary binding sites to miR-196a miR-148a which inhibit miR-196a and miR-148aexpression. The sequence of miR-196a and miR-148asensor wrere synthetised by GenScript (Shanghai, China). We inserted miR-196a miR-148a sensor into the 3' end of GFP, which generated GFP- miR-196a miR-148a sensor.