Part:BBa_K2908667

yCD

This figure shows the crystal structure of yeast cytosine deaminase (yCD).
The cytosine deaminase (CD)/5-fluorocytosine (5-FC) approach is the next most widely studied suicide gene therapy approach. CD is uniquely expressed in certain fungi and bacteria and it converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 854
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 466
  Illegal BsaI.rc site found at 503