Protein_Domain

Part:BBa_K2908667:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-16)
Revision as of 21:10, 17 October 2019 by Sqx98625 (Talk | contribs) (→‎References)


yCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 854
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 466
    Illegal BsaI.rc site found at 503


Design Notes

We must use the sequence of it correctly or yCD cannot work effectively.


Source

We get the sequence from NCBI and synthesized it by company.

References

Ko, T.-P., Lin, J.-J., Hu, C.-Y., Hsu, Y.-H., Wang, A.H.-J., Liaw, S.-H, Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution, (2003) J.Biol.Chem. 278: 19111-19117.