Coding

Part:BBa_K3195002

Designed by: JingYuan Liu   Group: iGEM19_SEU-Nanjing-China   (2019-08-22)
Revision as of 15:59, 17 October 2019 by Bread (Talk | contribs)


GST tagged carboxypeptidase Z/N Carboxypeptidase is a class of exopeptidases that can specifically degrade a peptide from its C-terminal of and release free amino acids. In our project, we found Carboxypeptidase Z/N can decompose microcystin RR with another enzyme called subtilisin-like protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
    Illegal XhoI site found at 1939
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//function/degradation
Parameters
originBranchiostoma belcheri tsingtauense
tagGST Tag