Reporter
ECFP

Part:BBa_E0022

Designed by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman   Group: Antiquity   (2003-01-31)
Revision as of 02:14, 17 October 2019 by Still Water (Talk | contribs)

enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


ECUST_China 2019 Charaterization

In ECUST_China 2019 characterization，we cloned pLAC to pet-28b and added eCFP as the reporter.

[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
abs
biology
emissionCFP
emit476
excitation
excite439
lum
proteinECFP-LVA
tagLVA