Part:BBa_K141003

Ucp 76 deleted

The uncoupling protein UCP 76 deleted is a proton carrier which is obtaines from the direct mugenesis of the Ucp1 gene. Ucp 76 has a deletion in the triplet the encodes for the Glycine 76. The mutant shows a generation time and an heat up capacity higher than UCP1. UCP 76 deleted uncouples the respiratory chain of ATP production, converting the metabolic energy in heat. The mutant shows a generation time and an heat up capacity higher than UCP1.

UCP 76 deleted is a 33kd protein. The protein has a tripartite structure. The structure displays an around 100 residues region which is three times repeated. Each part encodes for two transmembrane segments and one long hydrophilic loop.The functional carrier unit is an homodimer.

The main difference between UCP 76 deleted and most of the proteins with a nuclear codification is the lack of the importation targeting to the mitochondria in UCP 76 deleted proteins.

The condition that determines the mitochondria as the protein target lays in the first loop which protudes in the mitochondrial matrix.

The second loop of the matrix is essential for the insertion of the protein in the inner mitochondrial matrix.

That protein is without direct regulation and its generation time depends on the inner activity the protein.

Graphic about the growth of four different strains of Saccharomyces cerevisiae transformed with four kinds of UCP genes:

Y axis = Population in OD (optic density) units. X axis = Time in hours.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 47
  Illegal BglII site found at 344
  Illegal BamHI site found at 832
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 58
  
• 1000
  COMPATIBLE WITH RFC[1000]