Composite

Part:BBa_K3102040:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 10:13, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)

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T7-RBS-MDH-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the MDH sequence with an Adenine into Guanine at the 786bp position (A786G) because of the EcoRI enzyme. (BBa_K3102018)

Source

This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Sutherland P. et al., "Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.", J. Bacteriology, 163:1074-1079(1985)v