Coding

Part:BBa_K3102018:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 10:12, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)

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MDH (Malate dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated an Adenine into Guanine at the 786bp position (A786G) because of the EcoRI enzyme.

Source

This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721


References

Sutherland P. et al., "Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.", J. Bacteriology, 163:1074-1079(1985)v