Coding

Part:BBa_K3102019:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 09:19, 16 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)


DLD-II operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4330
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Cysteine into Thymine and a Guanine into Adenine at the 471bp and 476bp position (C471T & G476A), respectively, because of the PstI enzyme.

We also mutated a Adenine into Guanine and a Cysteine into Thymine at the 2842bp and 2845bp position (A2842G & C2845T), respectively, because of the EcoRI enzyme.

Source

This sequence comes from Shewanella Oneidensis was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-1405-MONOMER#tab=TU


References

Kouzuma A, Kasai T, Hirose A, Watanabe K. "Catabolic and regulatory systems in shewanella oneidensis MR-1 involved in electricity generation in microbial fuel cells". Front Microbiol. 2015;6(JUN):1–11.