Coding

Part:BBa_K2942704:Design

Designed by: Yihui Wang   Group: iGEM19_SYSU-Medicine   (2019-10-16)
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Carboxypeptidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 610
    Illegal NgoMIV site found at 808
    Illegal NgoMIV site found at 1237
    Illegal AgeI site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 160


Design Notes

We put this sequence at the downstream of the riboswitch to control its expression and we should make the number of the sequence base multiple of three to avoiding frameshift.


Source

Artificially Synthesized

References