Coding
Part:BBa_K2942704
Designed by: Yihui Wang Group: iGEM19_SYSU-Medicine (2019-10-16)
Carboxypeptidase
Carboxypeptidase is a kind of peptidase that specifically degrades and releases free amino acids from the C end of the peptide chain. And the ligand we use in our project is G2 type which could cleave C-terminal Glu under N-acetyl-L-aspartyl-L-glutamate structure. In our project we also carried out human codon optimization on the original CPG2 and added flag tag for labeling to increase protein expression and make detection easier.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1304
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 610
Illegal NgoMIV site found at 808
Illegal NgoMIV site found at 1237
Illegal AgeI site found at 1318 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 160
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Categories
Parameters
None |