Coding

Part:BBa_K2942704

Designed by: Yihui Wang   Group: iGEM19_SYSU-Medicine   (2019-10-16)
Revision as of 06:01, 16 October 2019 by Registry (Talk | contribs)

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Carboxypeptidase

Carboxypeptidase is a kind of peptidase that specifically degrades and releases free amino acids from the C end of the peptide chain. And the ligand we use in our project is G2 type which could cleave C-terminal Glu under N-acetyl-L-aspartyl-L-glutamate structure. In our project we also carried out human codon optimization on the original CPG2 and added flag tag for labeling to increase protein expression and make detection easier.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 610
    Illegal NgoMIV site found at 808
    Illegal NgoMIV site found at 1237
    Illegal AgeI site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 160


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Parameters
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