Composite

Part:BBa_K2913007

Designed by: Yaru Gu   Group: iGEM19_NEFU_China   (2019-10-15)
Revision as of 20:21, 15 October 2019 by Registry (Talk | contribs)

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Phuc3, one of the series of Phuc

This composite part consists of Pluc BBa_K2913003, conjugation sequence BBa_K2913002, and HucO sequence BBa_K2913001. Phuc3 includes two hucR binding site both in the Pluc and HucO. In the absence of uric acid (or xuanthine), HucR protein binds to Pluc as well as HucO and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-Pluc&HucR binding, RNA polymerase initiates a transcriptional process of downstream genes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None