Composite
Part:BBa_K2913005
Designed by: Yaru Gu Group: iGEM19_NEFU_China (2019-10-15)
Phuc1, one of the series of Phuc
This composite part consists of Pluc BBa_K2913003, conjugation sequence BBa_K2913002, and a random sequence as long as HucO sequence. Phuc1 includes one hucR binding site inside the Pluc. In the absence of uric acid (or xuanthine), HucR protein binds to Pluc and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-Pluc binding, RNA polymerase initiates a transcriptional process of downstream genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |