Coding

Part:BBa_K3209000

Designed by: SUN YUHAN   Group: iGEM19_JNFLS   (2019-10-15)
Revision as of 15:44, 15 October 2019 by Registry (Talk | contribs)

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Benzoylformate decarboxylase mutant (BFD-M7)

This is benzoylformate decarboxylase mutant(BFD-M7) from Pseudomonas putida. It contains 7 amino acids mutations, which is different from BFD (BBa_K2155001). The mutation information of BFD-M7 is as followings: W86R, N87T, L109G, L110E, H281V, Q282F and A460M. It can catalyze glycolaldehyde formation from formaldehye, then the glycolaldehyde reacts with formaldehyde to form dihydroxy acetone, also catalyzed by this enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 771
    Illegal NgoMIV site found at 1233
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


[edit]
Categories
//awards/basic_part/nominee
Parameters
None