Part:BBa_K2624000
hTERT promoter
It's the core promoter of human telomerase reverse transcriptase (hTERT) gene. And it's cancer-specific. If you want to do something specifically to cancer cells, for them to express certain product, you may need it.
Usage
The 2018 CPU_CHINA project is an AND-gated system targeting hepatocellular carcinoma. HTERT is one of the most known cancer-specific promoters, suitable for many cancer cell-related scheme. With this promoter, it’s more likely that normal cells would not express the protein contents that come after it. Only tumor cells (malignant ones especially) express NS5B and ‘open’ the AND gate, releasing miRNA that damage themselves. aaaa
Biology
Telomerase activation maintains telomeres and is critical for human carcinogenesis. Telomerase is a reverse transcriptase enzyme that carries its own RNA molecule. Transcriptional regulation of the enzyme part (hTERT) gene has been found to be the major mechanism for cancer-specific activation of telomerase. A number of factors have been identified to directly or indirectly regulate the hTERT promoter, which include famous cellular transcriptional activators such as c-Myc, Sp1, HIF-1, AP2, ER, as well as the repressors p53, WT1, and Menin, most of which comprise tumor suppressor gene products.
The chromatin structure via the DNA methylation or modulation of nucleosome histones is also important for regulation of the hTERT promoter. DNA unmethylation or histone methylation around the transcription start site of the hTERT promoter triggers the recruitment of histone acetyltransferase (HAT) activity, allowing hTERT transcription.
Results
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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File:Https://2019.igem.org/wiki/images/b/bc/T--CSU CHINA--Part BBA.jpg Fig. 1: Strength of the CMV promoter in different cell lines in REU. [http://2009.igem.org/Team:Heidelberg/Project_Measurement Relative Expression Units (REU)] were determined by three independent flow cytometry measurements on three different days. This measurement was confirmed by an independent technique - image analysis. |
 Figure 2: CMV strength changes depending on conditions As [http://2009.igem.org/Team:Heidelberg/Project_Measurement#Identification_of_challenges_unique_to_higher_eukaryotes there are no truly constitutive promoters in mammalian cells], both the reference promoter (Part:BBa_K203112) and CMV fluctuate dependent on condition (Erolimus induces extreme starvation). Measured by Flow cytometry 20 hours after transfection (unless specified otherwise).
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