Composite

Part:BBa_K3110040

Designed by: Mrugank Dake, Amartya Pal   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 04:01, 15 October 2019 by Registry (Talk | contribs)

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lldRO1-J23117-lldRO2 Strong RBS sfGFP

lldRO1 and lldRO2 are the operator regions which bind to the regulator element lldR and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can efficiently discriminate cancer vs non-cancer microenvironments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


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