Composite
Part:BBa_K3110039
Designed by: Mrugank Dake Group: iGEM19_IISER_Tirupati (2019-10-14)
Weak Promoter Weak RBS lldP+lldR+lldD
L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) and the regulator element (lldR) under the control of a weak promoter and a weak RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 327
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3649
Illegal AgeI site found at 463
Illegal AgeI site found at 926
Illegal AgeI site found at 1364
Illegal AgeI site found at 1523
Illegal AgeI site found at 1535
Illegal AgeI site found at 2281 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3086
[edit]
Categories
Parameters
None |