File:CharacK731520.jpg
Revision as of 15:28, 14 October 2019 by Katya (Talk | contribs) (Figure 1. iGEM Strasbourg team establish the kinetics of LacI-LacIQ promoter (BBa_K731300) induction by measuring GFP expression (BBa_E0040). Three different clones of XL1 Blue cells containing BBa_K731520 part were incubated overnight under rotation a...)
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Figure 1. iGEM Strasbourg team establish the kinetics of LacI-LacIQ promoter (BBa_K731300) induction by measuring GFP expression (BBa_E0040). Three different clones of XL1 Blue cells containing BBa_K731520 part were incubated overnight under rotation at 37° in LB media with supplementary 2% D-Glucose. After 18h the OD600 was adjust to OD = 0,1. The cells were incubated in presence of a different concentration of IPTG (0 μM, 20 μM, 100 μM and 500 μM) and PheraStar Plus BM6 LABTECH was used for real-time GFP measure. LB media was used as blanc. Bacterial cells not expressing GFP was used as the negative control. n = 3.
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| current | 15:28, 14 October 2019 | | 943 × 639 (93 KB) | Katya (Talk | contribs) | Figure 1. iGEM Strasbourg team establish the kinetics of LacI-LacIQ promoter (BBa_K731300) induction by measuring GFP expression (BBa_E0040). Three different clones of XL1 Blue cells containing BBa_K731520 part were incubated overnight under rotation a... |
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