Coding

Part:BBa_K3279006

Designed by: Liang Siwen   Group: iGEM19_CAU_China   (2019-10-14)
Revision as of 12:28, 14 October 2019 by Registry (Talk | contribs)

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INP-N ( N-terminus of Ice-nucleation protein ) from Pseudomonas syringae

Ice-nucleation protein (INP) is a secreted outer membrane protein, which is widely distributed in Pseudomonas syringae, Pseudomonas fluorescens and other Gram-negative bacteria. Compared with other surface carrier proteins, ice-nucleation protein has the advantage of stably expressing heterogenous proteins and displaying proteins with larger molecular weight. This part includes the sequence of INP-N (N terminus of Ice-nucleation protein), which is much shorter than INP and INP-NC. To allow this part fusion with other protein, we added an additional linker sequence at the 3'.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None