Coding

Part:BBa_K3076100:Design

Designed by: Choi Jonas Sze Ngo   Group: iGEM19_Hong_Kong_JSS   (2019-10-04)
Revision as of 13:41, 13 October 2019 by Pok430 (Talk | contribs)


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Source

The CgMT sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design Notes

There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine. When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064