Composite

Part:BBa_K2922022

Designed by: Xv Jiang   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 12:15, 13 October 2019 by Registry (Talk | contribs)

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T7-RBS-cex-T7-RBS-bgl1A functioned in Kil secretion cassette with promoter J23109

This part contains the sequence for the protein kil regulated by constitutive promoter J23109 and the sequence for the protein exoglucanase and beta-D-glucosidase regulated by T7 promoter. We used this part to achieve the secretion of exoglucanase and the expression of beta-D- Glucosidase with the function of kil secretion cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 818
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 3014
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 871
    Illegal SapI.rc site found at 954


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Parameters
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