Part:BBa_K2947001
UGT33
UDP-glucose-4-O-glucosyltransferase(T8GT) protein coding region.UGT33 is the most active gene among R. rosea Tyrosol-Modifying UGTs. The protein it encodes reduces tyrosol to produce salidroside. UGT33 is used as the last gene to complete salidroside biosynthesis from tyrosine.
Usage and Biology
The UGT super-family is one of the largest enzyme families in the plant kingdom. Previous research has shown that(Michel et al., 2017) among all the genes encoding UDP-glucose-4-O-glucosyltransferase(T8GT), UGT33 is the most active T8GT.
When 2019 GDSYZX is trying to transfer UGT33 to vector, the transfer of UGT33 into carrier was not smooth during the experiment. We started with the traditional enzyme training but it didn't work. Then we switched to a ligase, and it still was a false positive. Finally, we used the recombination link approach and successfully transferred UGT33 into the plasmid.
The protoplast and salidroside standard samples of arabidopsis thaliana transfected with UGT33 and 4HPAAS genes were compared by liquid chromatography. The peak value of the product measured in sample No. 1 was basically consistent with the peak value of salidroside standard samples. Therefore, we can determine that the transfected protoplast of arabidopsis thaliana can produce salidroside. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 624
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 34
Illegal SapI site found at 460
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