Composite

Part:BBa_K3088003

Designed by: Syailendra Karuna Sugito, Eko Ngadiono, Refael Alfa Budiman, Aditya Parawangsa   Group: iGEM19_UI_Indonesia   (2019-10-08)
Revision as of 11:30, 8 October 2019 by Registry (Talk | contribs)

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DiphTox + RFP

This design is an improvement of the previous DiphTox (DT) (BBa_K2607000). We changed the Ribosomal Binding Site and added Red Fluorescent Protein (RFP) sequence following the DT protein. The addition of RFP may ease and improve post-transformation bacterial selection process in addition to antibiotic selection to prevent selecting bacteria that contains re-ligated plasmid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 791
    Illegal AgeI site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None