Part:BBa_K3102021

ACS (Acetyl-coenzyme A synthetase) Escherichia.coli

ACS (Acetyl-coenzyme A synthetase) activity constitutes one of two distinct pathways by which E. coli activates acetate to acetyl-CoA in an ATP-dependent manner. [Brown et al., 1997; Kumari et al., 1995] The ACS pathway functions in a mainly anabolic role, scavenging acetate present in the extracellular medium. The ACS gene encoding this enzyme is overexpressed to enhance acetyl-CoA's generation.

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.

Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 373
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 802
  
• 1000
  COMPATIBLE WITH RFC[1000]